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KMID : 1059519930370100874
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Journal of the Korean Chemical Society
1993 Volume.37 No. 10 p.874 ~ p.880
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Analytical Studies of H2O2-Producing Oxidase Systems
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Hahn Young-hee
Cho Hae-Lim
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Abstract
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Spectrophotometric enzymatic analysis and amperometric enzymatic analysis for the determinations of glucose and ethanol were studied utilizing glucose oxidase (GO) and alcohol oxidase (AO), respectively, which commonly consume O2 and produce H2O2. For the determination of glucose, H2O2 were coupled to K4Fe(CN)6 via peroxidase producing K3Fe(CN)6 whose absorbance was measured at 418 nm or whose diffusion current was measured on the glassy carbon electrode at an applied potential of -55 mV vs. Ag/AgCl (sat. KCl) reference electrode. Amperometric analysis was 1000 times more sensitive as well as 10 times better in the linear concentration range than spectrophotometric analysis. For the determination of ethanol, AO only was used for the enzymatic analysis, since K3Fe(CN)6 was completely disappeared as soon as AO was added. Either rate of H2O2 produced was amperometrically measured at ＋0.900 V or rate of O2 consumed was measured at -0.500 V vs. Ag/AgCl(sat. KCl) reference electrode.
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